20.2 Cultivation and Processing of Microalgae
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water expulsion from the item is basic in any conversations about enormous scope
fuel creation. The solar drying process may be used for water removal but has a
serious concern of large land requirement for drying which thus makes it unfit for
large-scale processes [8].
20.2.1
Cultivation of Microalgae
It is very important to isolate and cultivate the microalgal species for biofuel produc-
tion. Cells are segregated and grown in appropriate surroundings. The method of
cell culture consists of various steps for a long-lasting period. The culture conditions
should be similar to that of the natural environment. The cultivation of microalgae
is done in the following sequence.
20.2.1.1
Isolation of Cell Cultures
The sample collection is the initial procedure followed by isolation of the required
cell. The growth of these green algae requires simple elements like light, water,
carbon source, temperature, and fewer macronutrients. These conditions are more
prevalent in coastal and oceanic sites and some from rock pools or even shorelines.
These samples are collected from these sites. Samples from these sites are collected
in separate bottles consisting of a cylindrical tube with stoppers at the ends; it may
be fitted with a closing device activated by a messenger. The microalgae samples
that are collected need to be handled gently with care. These have to be transferred
rapidly because the damaged and dry cells cannot produce viable cultures. The
collection details including date, location, date, depth of sample from where they
are collected, water temperature, etc., must be recorded for references. The collected
samples may then be transported in small plastic water bottles. Before the bottle
is filled, it may be rinsed with the sample water to avoid the bubbles. The bottle
containing samples must be well insulated.
20.2.1.2
Single-Cell Isolation
The micropipette is used to isolate the single cell. It is done to remove a particular cell
from the water sample without damage and put the same into a germ-free droplet
of growth medium. The sterile droplet consists of the target cell. Optimum culture
medium for algal growth has to be established as the newly isolated strain will be
difficult for maintenance. The isolates can be put into several culture media and
their growths are monitored. Algal growth is monitored regularly under microscopic
examination. If the culture begins to grow, it has to be relocated to a glass flask or
culture vessel. On establishing the culture, it may be grown in huge volumes. Other
isolation methods are also used by different researchers. They include the use of
agar, centrifugation, flow cytometry, dilution techniques, and photo axis.
20.2.2
Techniques
20.2.2.1
Filtration
Filtration is the most appropriate method for sterilization of the samples of smaller
quantities and heat-sensitive elements like vitamins. Membrane filters may be used